To support the growing field of microbiome research, Pine Lake Laboratories has developed an assay for short chain fatty acids (acetic, butyric, and propionic acid) in human plasma. These targeted metabolites can be used as an indicator of microbiome activity in research subjects and as biomarkers to evaluate the effect of various pharmaceutical drug treatments during clinical trials. The assay uses GC-MS and can reach an LOQ of 1.00 ug/mL. for each short chain fatty acid.